TAT peptide

生物活性

TAT peptide is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1][2].

体外研究(In Vitro)

TAT peptide is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1]. TAT peptide (GRKKRRQRRRPQ) functionalized hybrid nanoparticles are also studied due to their combined magnetic enrichment and optical detection for cell separation and rapid cell labelling. A cell viability assay reveals good biocompatibility of these hybrid nanoparticles[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

TAT peptide 相关抗体:

分子量

1621.91

Formula

C65H124N34O15

性状

固体

颜色

White to off-white

Sequence

Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Gln

Sequence Shortening

GRKKRRQRRRPQ

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder

-80°C

2 years

-20°C

1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

细胞实验：

H2O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

Contents

Assign value

Acidic amino acid

Asp (D), Glu (E), and the C-terminal -COOH.

-1

Basic amino acid

Arg (R), Lys (K), His (H), and the N-terminal -NH2

+1

Neutral amino acid

Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)

0

3. Recommended solution:

Overall charge of peptide

Details

Negative (<0)

1. Try to dissolve the peptide in water first.

2. If water fails, add NH4OH (<50 μL).

3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.

Positive (>0)

1. Try to dissolve the peptide in water first.

2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.

3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.

Zero (=0)

1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.

2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

纯度 & 产品资料

Data Sheet (514 KB)

SDS (135 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Orzáez M, et al. Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells toerlotinib/cell-cycle inhibitors combination treatment. Cell Death Dis. 2012 Oct 25;3:e415.

[Content Brief]

[2]. Lou L, et al. Functionalized magnetic-fluorescent hybrid nanoparticles for cell labelling. Nanoscale. 2011 May;3(5):2315-23.

[Content Brief]